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Peptides / Peptides / Healing & Recovery

NAD+

Core Cellular Coenzyme and Metabolic Regulator for In Vitro and In Vivo Research

NAD+

Nicotinamide Adenine Dinucleotide (NAD+) is a fundamental coenzyme present in all living cells, serving as a critical cofactor in both cellular oxidation-reduction reactions and non-redox signaling pathways. In research settings, it is actively investigated for its role in cellular respiration, sirtuin activation, DNA repair, and its potential to counteract age-related metabolic and neurodegenerative decline.

Typical dose
Highly dependent on model organism; typically 10–50 mg/kg via intranasal or parenteral routes in rodent assays.
Half-life
Extremely rapid in plasma (approximately 2 to 15 minutes due to rapid enzymatic clearance by CD38 and ectoenzymes)
Administration
Intranasal administration (via liquid spray formulations), Intravenous (IV), Intraperitoneal (IP) infusion
Research status
Preclinical / research use only
Calculate a dose

What it is

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme central to cellular energy metabolism, mitochondrial function, and DNA repair. Injectable NAD+ is studied for effects on fatigue, recovery, and metabolic drive.

Why researchers use it

  • Supports mitochondrial ATP production
  • Studied for cognitive clarity and energy
  • Investigated in aging biology and neuroprotection contexts

Dosing protocol (for research)

  • Common: 100–500 mg per session
  • Higher doses (up to 2,000 mg) have been studied but are less common
  • Subcutaneous or intramuscular; IV pushes exist but require clinical setting

Preparation & injection

  • Draw slowly and inject slowly — rapid administration is associated with flushing, chest pressure, and cramping
  • Warm the vial to body temperature to reduce injection sting
  • Rotate injection sites to avoid soreness

Safety notes

  • Rapid dosing can cause transient flushing, nausea, and chest discomfort — slow the push
  • Stay well hydrated before and after
  • Long-term high-dose data is limited

Reconstitution & concentration

Standard vial: 500 mg NAD+ per vial, typically reconstituted or diluted per supplier instructions. Research protocols vary widely.

  • Low-dose IM/SubQ: 50–100 mg daily or several times weekly
  • Cycle length: highly variable, weeks to months depending on study goals

Inject slowly — researchers warn that rapid administration can cause flushing, nausea, cramping, or chest tightness. Maintain hydration and electrolyte balance.

Stacks

  • Core Energy Restoration Stack: NAD+ 250–500 mg IM 1–2×/week + L-Carnitine 500–1,000 mg IM/SubQ daily or every other day
  • Performance Recovery Stack: Super Human 1–2 mL IM 2–3×/week + NAD+ 250–500 mg IM weekly
  • Metabolic Drive Stack: SLU-PP-332 250 mcg oral daily + NAD+ 250–500 mg IM weekly
  • Advanced Recovery Protocol: KLOW 0.2–0.4 mL SubQ daily + NAD+ 250–500 mg IM weekly + L-Carnitine 500–1,000 mg IM/SubQ daily

Researcher tips also suggest pairing NAD+ with mitochondrial cofactors — CoQ10, L-Carnitine, or Alpha-Lipoic Acid — to amplify results.

Where to source